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Title: :  MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM
PaperId: :  11820
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 3 Issue 3 2017
DUI:    16.0415/IJARIIE-11820
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Dilip JadhaoInstitute of Chemical Technology (Formerly UDCT), N. P. Road, Matunga (E), Mumbai 400 019, India.
Girish KambleShri R. R. Lahoti Science College, Morshi. Dist. Amravati (M.S.),444 905 India.
Ravi DhandeShri R. R. Lahoti Science College, Morshi. Dist. Amravati (M.S.),444 905 India.

Abstract

Phyto-chemistry
Punicagrantum, ellagic acid, HPLC, isolation.
A simple isolation method and rapid, specific high-performance liquid chromatographic method was developed and validated for Ellagic acid from Punica grantum extract. HPLC analysis was performed on C18 column using a 90:10 (v/v) mixtures of acetonitrile and methanol as isocratic mobile phase at flow rate 1.0 ml/min. UV detection was at 254 nm for filixic acid PBP. Filixic acid shown retention time at 4.02 min. Method was validated for accuracy, precision, linearity, specificity and sensitivity in accordance with International Conference on Harmonisation guidelines. Validation studies revealed that the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficient (r2 > 0.993) was obtained for calibration plots in the range tested. The limit of detection was 2.25 μg/ml and limit of quantification was 7.53 μg/ml for ellagic acid. Intra and inter-day relative standard deviation of precision was less than 1.50 %. Recovery was between 94.57 and 101.05 % ellagic acid. The method can be successfully used for quantitative analysis of ellagic acid in Punica grantum for day-to-day studies.

Citations

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IJARIIE Dilip Jadhao, Girish Kamble, and Ravi Dhande. "MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM" International Journal Of Advance Research And Innovative Ideas In Education Volume 3 Issue 3 2017 Page 4205-4210
MLA Dilip Jadhao, Girish Kamble, and Ravi Dhande. "MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM." International Journal Of Advance Research And Innovative Ideas In Education 3.3(2017) : 4205-4210.
APA Dilip Jadhao, Girish Kamble, & Ravi Dhande. (2017). MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM. International Journal Of Advance Research And Innovative Ideas In Education, 3(3), 4205-4210.
Chicago Dilip Jadhao, Girish Kamble, and Ravi Dhande. "MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM." International Journal Of Advance Research And Innovative Ideas In Education 3, no. 3 (2017) : 4205-4210.
Oxford Dilip Jadhao, Girish Kamble, and Ravi Dhande. 'MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM', International Journal Of Advance Research And Innovative Ideas In Education, vol. 3, no. 3, 2017, p. 4205-4210. Available from IJARIIE, http://ijariie.com/AdminUploadPdf/MODIFICATION_AND_VALIDITATION_OF_HPLC_METHOD_FOR_ISOLATION_OF_ELLAGIC_ACID_FROM_PUNICA_GRANTUM_ijariie11820.pdf (Accessed : 21 April 2020).
Harvard Dilip Jadhao, Girish Kamble, and Ravi Dhande. (2017) 'MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM', International Journal Of Advance Research And Innovative Ideas In Education, 3(3), pp. 4205-4210IJARIIE [Online]. Available at: http://ijariie.com/AdminUploadPdf/MODIFICATION_AND_VALIDITATION_OF_HPLC_METHOD_FOR_ISOLATION_OF_ELLAGIC_ACID_FROM_PUNICA_GRANTUM_ijariie11820.pdf (Accessed : 21 April 2020)
IEEE Dilip Jadhao, Girish Kamble, and Ravi Dhande, "MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM," International Journal Of Advance Research And Innovative Ideas In Education, vol. 3, no. 3, pp. 4205-4210, May-Jun 2017. [Online]. Available: http://ijariie.com/AdminUploadPdf/MODIFICATION_AND_VALIDITATION_OF_HPLC_METHOD_FOR_ISOLATION_OF_ELLAGIC_ACID_FROM_PUNICA_GRANTUM_ijariie11820.pdf [Accessed : 21 April 2020].
Turabian Dilip Jadhao, Girish Kamble, and Ravi Dhande. "MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 3 number 3 (21 April 2020).
Vancouver Dilip Jadhao, Girish Kamble, and Ravi Dhande. MODIFICATION AND VALIDITATION OF HPLC METHOD FOR ISOLATION OF ELLAGIC ACID FROM PUNICA GRANTUM. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2017 [Cited : 21 April 2020]; 3(3) : 4205-4210. Available from: http://ijariie.com/AdminUploadPdf/MODIFICATION_AND_VALIDITATION_OF_HPLC_METHOD_FOR_ISOLATION_OF_ELLAGIC_ACID_FROM_PUNICA_GRANTUM_ijariie11820.pdf
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