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Title: :  The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film
PaperId: :  3078
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 2 Issue 5 2016
DUI:    16.0415/IJARIIE-3078
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
SURESH MOREJAIHIND POLYTECHNIC KURAN ,PUNE
kiran paithankarJAIHIND POLYTECHNIC KURAN ,PUNE
sunil mirganeDepartment of Chemistry, JES Art, Science and Commerce College Jalna, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
vikas gadeDepartment of Physics, Shri Anand College Pathardi, Savitribai Phule University, Pune, India

Abstract

APPLIED CHEMISTRY
Urea, Polymer, Biosensor, Enzyme, BSA, Peptide linkage, PANI 2AP.
A Potentiometric biosensor based on bovine serum albumin (BSA) embedded surface modified PANI/2AP has been developed for the detection of urea in water sample. The enzyme, urease (Urs), was covalently linked to free amino groups present over the BSA embedded modified surface of the conducting PANI/2AP composite film electrochemically deposited onto an indium–tin-oxide (ITO) film. The biosensor has been characterized by UV–visible, infrared spectroscopy and SEM. Potentiometric response of the enzyme electrode (Urs/BSA- PANI/2AP /ITO) were measured as a function of urea concentration in phosphate buffer. It has been found that the electrode responds to low urea concentration with wider range of detection. The electrode showed a linear response range of current 0-30µA for 1-100mM solution of urea in PBS at pH 7.0. The response time is about 50-70 s reaching to a 90% steady-state potential value. These results indicate an efficient covalent linkage of enzyme to free amino groups of the BSA molecules over the surface of PANI/2AP composite film, which leads to high enzyme loading, an increased lifetime stability of the electrode and an improved wide range of detection of low urea concentration in aqueous medium.

Citations

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IJARIIE SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. "The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film" International Journal Of Advance Research And Innovative Ideas In Education Volume 2 Issue 5 2016 Page 147-154
MLA SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. "The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film." International Journal Of Advance Research And Innovative Ideas In Education 2.5(2016) : 147-154.
APA SURESH MORE, kiran paithankar, sunil mirgane, & vikas gade. (2016). The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film. International Journal Of Advance Research And Innovative Ideas In Education, 2(5), 147-154.
Chicago SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. "The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film." International Journal Of Advance Research And Innovative Ideas In Education 2, no. 5 (2016) : 147-154.
Oxford SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. 'The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film', International Journal Of Advance Research And Innovative Ideas In Education, vol. 2, no. 5, 2016, p. 147-154. Available from IJARIIE, http://ijariie.com/AdminUploadPdf/The_Development_of_A_Urea_Biosensor_Based_on_BSA_Embedded_Surface_Modified_PANI_2AP_composite_Film_ijariie3078.pdf (Accessed : 18 December 2017).
Harvard SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. (2016) 'The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film', International Journal Of Advance Research And Innovative Ideas In Education, 2(5), pp. 147-154IJARIIE [Online]. Available at: http://ijariie.com/AdminUploadPdf/The_Development_of_A_Urea_Biosensor_Based_on_BSA_Embedded_Surface_Modified_PANI_2AP_composite_Film_ijariie3078.pdf (Accessed : 18 December 2017)
IEEE SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade, "The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film," International Journal Of Advance Research And Innovative Ideas In Education, vol. 2, no. 5, pp. 147-154, Sep-Oct 2016. [Online]. Available: http://ijariie.com/AdminUploadPdf/The_Development_of_A_Urea_Biosensor_Based_on_BSA_Embedded_Surface_Modified_PANI_2AP_composite_Film_ijariie3078.pdf [Accessed : 18 December 2017].
Turabian SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. "The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 2 number 5 (18 December 2017).
Vancouver SURESH MORE, kiran paithankar, sunil mirgane, and vikas gade. The Development of A Urea Biosensor Based on BSA Embedded Surface Modified PANI/2AP composite Film. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2016 [Cited : 18 December 2017]; 2(5) : 147-154. Available from: http://ijariie.com/AdminUploadPdf/The_Development_of_A_Urea_Biosensor_Based_on_BSA_Embedded_Surface_Modified_PANI_2AP_composite_Film_ijariie3078.pdf
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