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Title: :  Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism
PaperId: :  1642
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 2 Issue 2 2016
DUI:    16.0415/IJARIIE-1642
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
S.VELUMANIPROF. DHANAPALAN COLLEGE OF ARTS AND SCIENCE

Abstract

BIOTECHNOLOGY
Fibrin, Fibrinolytic activity, PIB, Affinity chromatography.
Fibrinolytic enzymes are agents that dissolve fibrin clots. These fibrinolytic agents have potential use to treat cardiovascular diseases, such as heart attack and stroke. The present study aimed at the production, purification and characterization of fibrinolytic enzyme from the bacteria isolated from sea water. The isolated organism was cultured in the Luria Bertani broth (LB broth) at 37ºC for 24 hrs. The extracellular enzyme was extracted and the assays like fibrinolytic, caesinolytic, albuminolytic activity was done. The fibrinolytic activity of that enzyme was twofold higher than that of caesinolytic activity and 10 fold higher than that of albuminolytic. The activity of the enzyme was also checked on RBC and on the animal tissue and no damage was occurred. The optimum pH and temperature were 7 and 45°C. Enzyme was purified using differential ammonium sulphate precipitation. The 75% saturation was found to be more active and it was further purified by using Affinity chromatography run in SDS-Polyacrylamide Gel Electrophoresis. It was characterized as metalloprotease by using protease inhibitors. The biochemical characterization of the organism was carried out and compared with the PIB (Probability Index of Bacteria )win matrix and finally it was identified as Vibrio campbelli.

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IJARIIE S.VELUMANI. "Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism" International Journal Of Advance Research And Innovative Ideas In Education Volume 2 Issue 2 2016 Page 1-10
MLA S.VELUMANI. "Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism." International Journal Of Advance Research And Innovative Ideas In Education 2.2(2016) : 1-10.
APA S.VELUMANI. (2016). Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism. International Journal Of Advance Research And Innovative Ideas In Education, 2(2), 1-10.
Chicago S.VELUMANI. "Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism." International Journal Of Advance Research And Innovative Ideas In Education 2, no. 2 (2016) : 1-10.
Oxford S.VELUMANI. 'Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism', International Journal Of Advance Research And Innovative Ideas In Education, vol. 2, no. 2, 2016, p. 1-10. Available from IJARIIE, http://ijariie.com/AdminUploadPdf/Isolation__Screening__Characterization_and_Production_of_Fibrinolytic_enzyme_from_marine_microorganism_ijariie1642.pdf (Accessed : 10 April 2019).
Harvard S.VELUMANI. (2016) 'Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism', International Journal Of Advance Research And Innovative Ideas In Education, 2(2), pp. 1-10IJARIIE [Online]. Available at: http://ijariie.com/AdminUploadPdf/Isolation__Screening__Characterization_and_Production_of_Fibrinolytic_enzyme_from_marine_microorganism_ijariie1642.pdf (Accessed : 10 April 2019)
IEEE S.VELUMANI, "Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism," International Journal Of Advance Research And Innovative Ideas In Education, vol. 2, no. 2, pp. 1-10, Mar-App 2016. [Online]. Available: http://ijariie.com/AdminUploadPdf/Isolation__Screening__Characterization_and_Production_of_Fibrinolytic_enzyme_from_marine_microorganism_ijariie1642.pdf [Accessed : 10 April 2019].
Turabian S.VELUMANI. "Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 2 number 2 (10 April 2019).
Vancouver S.VELUMANI. Isolation, Screening, Characterization and Production of Fibrinolytic enzyme from marine microorganism. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2016 [Cited : 10 April 2019]; 2(2) : 1-10. Available from: http://ijariie.com/AdminUploadPdf/Isolation__Screening__Characterization_and_Production_of_Fibrinolytic_enzyme_from_marine_microorganism_ijariie1642.pdf
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