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Title: :  Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken
PaperId: :  1173
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 1 Issue 3 2015
DUI:    16.0415/IJARIIE-1173
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Ahmed KhalidCollege of Animal Science and Technology, Northeast Agricultural University
Nagam khudhairKey Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University
Yu NaNortheast Agricultural University, Harbin
Zhang GuixueNortheast Agricultural University, Harbin

Abstract

Biotechnology
AA Broiler Chicken, Fibroblast cells, Froze Embryos, Fluorescent proteins and Karyotype
This study is to establish fibroblast line of AA broiler chicken from frozen embryos. According to the requirement of (ATCC) tests, the study depended on testing several points such as growth curve, cryopreservation, Karyotyping of chromosomes and protein translation of transfected vector. The results showed that the cells were morphologically consistent with fibroblasts and the growth curve was sigmoidal with a population doubling time (PDT) was(41.6h FE , 43.2h AFE). Cell viability was 91.8% before cryopreservation and 82.2% after thawing. Karyotyping for chromosome number was of 2n=78 and the rate of cellular diploid was 92.20±0.56% for Fresh Embryos (FE) and 91.42±0.23% After Freezing Embryos (AFE). The fluorescent protein was translated at 24h, 48 h and 72h after transfection and the colonies of fluorescent fibroblast cells were formed one months later. In conclusion, the procedure for cryopreservation and thawing of embryos as well as preparation of fibroblast cells were proven to be practicable. The fibroblast line of AA broiler from frozen embryos has been established and the valuable genetic resource of AA broiler chicken was well protected at the cellular level.

Citations

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IJARIIE Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. "Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken" International Journal Of Advance Research And Innovative Ideas In Education Volume 1 Issue 3 2015 Page 48-56
MLA Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. "Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken." International Journal Of Advance Research And Innovative Ideas In Education 1.3(2015) : 48-56.
APA Ahmed Khalid, Nagam khudhair, Yu Na, & Zhang Guixue. (2015). Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken. International Journal Of Advance Research And Innovative Ideas In Education, 1(3), 48-56.
Chicago Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. "Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken." International Journal Of Advance Research And Innovative Ideas In Education 1, no. 3 (2015) : 48-56.
Oxford Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. 'Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken', International Journal Of Advance Research And Innovative Ideas In Education, vol. 1, no. 3, 2015, p. 48-56. Available from IJARIIE, http://ijariie.com/AdminUploadPdf/Preparation_and_Cryopreserved_of_Fibroblasts_from_Frozen_Embryos_of__Arbor_Acre__Chicken_ijariie1173_volume_1_13_page_48_56.pdf (Accessed : 09 May 2018).
Harvard Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. (2015) 'Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken', International Journal Of Advance Research And Innovative Ideas In Education, 1(3), pp. 48-56IJARIIE [Online]. Available at: http://ijariie.com/AdminUploadPdf/Preparation_and_Cryopreserved_of_Fibroblasts_from_Frozen_Embryos_of__Arbor_Acre__Chicken_ijariie1173_volume_1_13_page_48_56.pdf (Accessed : 09 May 2018)
IEEE Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue, "Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken," International Journal Of Advance Research And Innovative Ideas In Education, vol. 1, no. 3, pp. 48-56, Jul-Aug 2015. [Online]. Available: http://ijariie.com/AdminUploadPdf/Preparation_and_Cryopreserved_of_Fibroblasts_from_Frozen_Embryos_of__Arbor_Acre__Chicken_ijariie1173_volume_1_13_page_48_56.pdf [Accessed : 09 May 2018].
Turabian Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. "Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 1 number 3 (09 May 2018).
Vancouver Ahmed Khalid, Nagam khudhair, Yu Na, and Zhang Guixue. Preparation and Cryopreserved of Fibroblasts from Frozen Embryos of (Arbor Acre) Chicken. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2015 [Cited : 09 May 2018]; 1(3) : 48-56. Available from: http://ijariie.com/AdminUploadPdf/Preparation_and_Cryopreserved_of_Fibroblasts_from_Frozen_Embryos_of__Arbor_Acre__Chicken_ijariie1173_volume_1_13_page_48_56.pdf
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