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Title: :  Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)
PaperId: :  1821
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 2 Issue 2 2016
DUI:    16.0415/IJARIIE-1821
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Naveen GauravAssistant Professor, Department of Biotechnology, S G R R P G College Dehradun, U.K.
A P SinghProfessor, Department of Botany Govt. P.G. Science College, Rewa, M.P.
Abhishekh SrivastavaAssistant Professor, Department of Botany Govt. S.V. College Teonthar, M.P.
Arun KumarAssistant Professor, Department of Biotechnology, S G R R P G College Dehradun, U.K.

Abstract

biological and medical science
Withania somnifera, Solanaceae, aromatic properties, in vitro microprogation, B5 medium, Sucrose etc.
Ashwagandha (Withania somnifera) belongs to the member of family Solanaceae, having enormous aromatic properties and medicinal properties, has been included in an ancient (early) text of Ayurveda. It is very useful as an, contraceptive, amoebocide, bactericide, abortifacient, anodyne, and diuretic. But the risks of fungal infections are very high in these plants. Due to its over use this plants is going towards extinction so in vitro microprogation is a best method to protect this plant as well as to produced value added compounds in a very short time without any external environmental hazards. Higher percentage of in vitro morphogenic response was exhibited by explants mature embryo followed by explants mature cotyledon leaves. Half B5 medium (gamborg medium) with NAA, IBA, 2,4- Di-chlorophenyl acetic acid (2,4-D), BAP, Kinetin (Kn) and Sucrose (5% w/v) was employed either separately or in mixture. Cotyledonary leaves are generally produces callus in callus induction media and after several sub culturing its produces shoots and roots in shoot and root induction media. Regenerated plantlets were obtained successfully in the field after hardening. Mostly for in vitro organogenesis MS medium has been employed bus it has been also done in B5 medium with cotyledonary leave's explants of Withania somnifera.

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IJARIIE Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. "Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)" International Journal Of Advance Research And Innovative Ideas In Education Volume 2 Issue 2 2016 Page 687-693
MLA Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. "Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)." International Journal Of Advance Research And Innovative Ideas In Education 2.2(2016) : 687-693.
APA Naveen Gaurav, A P Singh, Abhishekh Srivastava, & Arun Kumar. (2016). Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated). International Journal Of Advance Research And Innovative Ideas In Education, 2(2), 687-693.
Chicago Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. "Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)." International Journal Of Advance Research And Innovative Ideas In Education 2, no. 2 (2016) : 687-693.
Oxford Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. 'Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)', International Journal Of Advance Research And Innovative Ideas In Education, vol. 2, no. 2, 2016, p. 687-693. Available from IJARIIE, http://ijariie.com/AdminUploadPdf/Effect_of_Different_Auxins_Alone_and_Mixture_of_Benzene_ijariie1821.pdf (Accessed : ).
Harvard Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. (2016) 'Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)', International Journal Of Advance Research And Innovative Ideas In Education, 2(2), pp. 687-693IJARIIE [Online]. Available at: http://ijariie.com/AdminUploadPdf/Effect_of_Different_Auxins_Alone_and_Mixture_of_Benzene_ijariie1821.pdf (Accessed : )
IEEE Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar, "Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)," International Journal Of Advance Research And Innovative Ideas In Education, vol. 2, no. 2, pp. 687-693, Mar-App 2016. [Online]. Available: http://ijariie.com/AdminUploadPdf/Effect_of_Different_Auxins_Alone_and_Mixture_of_Benzene_ijariie1821.pdf [Accessed : ].
Turabian Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. "Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated)." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 2 number 2 ().
Vancouver Naveen Gaurav, A P Singh, Abhishekh Srivastava, and Arun Kumar. Effect of Different Auxins Alone and Mixture of Benzene Aminopurine (BAP) and Kinetin (Kn) Along with Auxin 2,4-D on In Vitro Growth and Multiplication of Callus in B5 Medium Derived from Embryonic Cotyledon Excised Explants of Ashwagandha (Cultivated). International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2016 [Cited : ]; 2(2) : 687-693. Available from: http://ijariie.com/AdminUploadPdf/Effect_of_Different_Auxins_Alone_and_Mixture_of_Benzene_ijariie1821.pdf
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