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Title: :  Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment
PaperId: :  11618
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 6 Issue 2 2020
DUI:    16.0415/IJARIIE-11618
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Govind G. DhageShri Vyankatesh Science College, Deulgaon Raja

Abstract

Microbiology
L-asparaginase, mesophillic, Bacillus sp
Bacterial L-asparaginase is amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukaemia. In the present study, alkaline isolates were screened for potential producers of L-asparaginase using L-asparagine major source in M9 medium and phenol red as indicator. Further the isolates were been studied morphologically, biochemically to identify Bacillus sp, a novel strain. It produced largest zone of hydrolysis due to the bacteria utilised aspargine as nitrogen source. Bacillus sp. was explored for the production of extra-cellular L-asparaginase enzyme. The enzyme production was carried out by submerged fermentation. The studied strains shows prominant enzyme activity as mesophillic Bacillus Sp. at pH 7.6 and temperature 700C with findings that metal ion Mg2+ as enhancer and EDTA as a inhibiter

Citations

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IJARIIE Govind G. Dhage. "Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment" International Journal Of Advance Research And Innovative Ideas In Education Volume 6 Issue 2 2020 Page 685-693
MLA Govind G. Dhage. "Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment." International Journal Of Advance Research And Innovative Ideas In Education 6.2(2020) : 685-693.
APA Govind G. Dhage. (2020). Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment. International Journal Of Advance Research And Innovative Ideas In Education, 6(2), 685-693.
Chicago Govind G. Dhage. "Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment." International Journal Of Advance Research And Innovative Ideas In Education 6, no. 2 (2020) : 685-693.
Oxford Govind G. Dhage. 'Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment', International Journal Of Advance Research And Innovative Ideas In Education, vol. 6, no. 2, 2020, p. 685-693. Available from IJARIIE, https://ijariie.com/AdminUploadPdf/Kinetic_Study_of_L_asparaginase_From_Alkaline_Bacillus_sp__Isolated_From_hypersaline_environment_ijariie11618.pdf (Accessed : ).
Harvard Govind G. Dhage. (2020) 'Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment', International Journal Of Advance Research And Innovative Ideas In Education, 6(2), pp. 685-693IJARIIE [Online]. Available at: https://ijariie.com/AdminUploadPdf/Kinetic_Study_of_L_asparaginase_From_Alkaline_Bacillus_sp__Isolated_From_hypersaline_environment_ijariie11618.pdf (Accessed : )
IEEE Govind G. Dhage, "Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment," International Journal Of Advance Research And Innovative Ideas In Education, vol. 6, no. 2, pp. 685-693, Mar-App 2020. [Online]. Available: https://ijariie.com/AdminUploadPdf/Kinetic_Study_of_L_asparaginase_From_Alkaline_Bacillus_sp__Isolated_From_hypersaline_environment_ijariie11618.pdf [Accessed : ].
Turabian Govind G. Dhage. "Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 6 number 2 ().
Vancouver Govind G. Dhage. Kinetic Study of L-asparaginase From Alkaline Bacillus sp. Isolated From hypersaline environment. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2020 [Cited : ]; 6(2) : 685-693. Available from: https://ijariie.com/AdminUploadPdf/Kinetic_Study_of_L_asparaginase_From_Alkaline_Bacillus_sp__Isolated_From_hypersaline_environment_ijariie11618.pdf
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