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Title: :  Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)
PaperId: :  21604
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 9 Issue 6 2023
DUI:    16.0415/IJARIIE-21604
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Dr. Ali Husein Taher AlhilaliIraqi ministry of agriculture

Abstract

agriculture
ethyl methane sulphonate, germination, inter-simple sequence repeats, mutation, seed, wheat
We conducted laboratory experiments using ethyl methane sulphonate (EMS) to induce mutation in wheat seeds. We subsequently performed inter-simple sequence repeats (ISSR) analysis to determine the mutational effects of EMS on seed germination and on the vigour index of wheat seeds. ISSR was assayed to determine genetic diversity using eight different concentrations of EMS) and 18 markers that enabled the treatment results to be compared. Among the 18 ISSR markers, marker 5 was associated with a high level of polymorphism (96.87%), whereas a lower level of polymorphism (32.81%) was associated with marker 16. All of the primers applied in the study enabled differentiation of the treatments. The results obtained using the primers numbered 3, 5, 7, 11, 12, 13, 15, 16 and 17 were satisfactory, whereas those obtained using the remaining nine primers were slightly better. There was a significant increase in the seed germination percentage (91%) at an EMS concentration of 1.50%, used in Treatment 7, with notable increases in the lengths of shoots (13 cm) and roots (9 cm). Moreover, at an EMS concentration of 1.50%, the fresh and dry weights of the seedlings increased significantly by 0.2353 g and 0.016 g, respectively. We conclude that the use of ISSR and ISSR markers to determine EMS mutagenesis is effective for analysing mutated wheat differentiation and for determining the effects of EMS on seed germination and the vigour index of wheat.

Citations

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IJARIIE Dr. Ali Husein Taher Alhilali. "Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)" International Journal Of Advance Research And Innovative Ideas In Education Volume 9 Issue 6 2023 Page 93-102
MLA Dr. Ali Husein Taher Alhilali. "Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)." International Journal Of Advance Research And Innovative Ideas In Education 9.6(2023) : 93-102.
APA Dr. Ali Husein Taher Alhilali. (2023). Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.). International Journal Of Advance Research And Innovative Ideas In Education, 9(6), 93-102.
Chicago Dr. Ali Husein Taher Alhilali. "Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)." International Journal Of Advance Research And Innovative Ideas In Education 9, no. 6 (2023) : 93-102.
Oxford Dr. Ali Husein Taher Alhilali. 'Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)', International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 6, 2023, p. 93-102. Available from IJARIIE, https://ijariie.com/AdminUploadPdf/Effects_of_Ethyl_Methane_Sulphonate_on_Seed_Germination_and_the_Vigour_Index_of_Wheat__Triticum_aestivum_L___ijariie21604.pdf (Accessed : 11 December 2023).
Harvard Dr. Ali Husein Taher Alhilali. (2023) 'Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)', International Journal Of Advance Research And Innovative Ideas In Education, 9(6), pp. 93-102IJARIIE [Online]. Available at: https://ijariie.com/AdminUploadPdf/Effects_of_Ethyl_Methane_Sulphonate_on_Seed_Germination_and_the_Vigour_Index_of_Wheat__Triticum_aestivum_L___ijariie21604.pdf (Accessed : 11 December 2023)
IEEE Dr. Ali Husein Taher Alhilali, "Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)," International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 6, pp. 93-102, Nov-Dec 2023. [Online]. Available: https://ijariie.com/AdminUploadPdf/Effects_of_Ethyl_Methane_Sulphonate_on_Seed_Germination_and_the_Vigour_Index_of_Wheat__Triticum_aestivum_L___ijariie21604.pdf [Accessed : 11 December 2023].
Turabian Dr. Ali Husein Taher Alhilali. "Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.)." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 9 number 6 (11 December 2023).
Vancouver Dr. Ali Husein Taher Alhilali. Effects of Ethyl Methane Sulphonate on Seed Germination and the Vigour Index of Wheat (Triticum aestivum L.). International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2023 [Cited : 11 December 2023]; 9(6) : 93-102. Available from: https://ijariie.com/AdminUploadPdf/Effects_of_Ethyl_Methane_Sulphonate_on_Seed_Germination_and_the_Vigour_Index_of_Wheat__Triticum_aestivum_L___ijariie21604.pdf
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