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Title: :  Expression of the srlI gene to improve salt tolerance in Escherichia coli
PaperId: :  21871
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 9 Issue 6 2023
DUI:    16.0415/IJARIIE-21871
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Ali Hussein Taher AlhilaliIraqi ministry of agriculture

Abstract

agriculture
E. coli top10, E. coli DH5 alpha, srlD gene
In trying to engineer E. coli to enhance salt tolerance, this study shows constitutive over-expression of the srlI (glucitol-6-phosphate dehydrogenase) gene studying GMO E. coli. These genes used by studied salinity stress at five concentrations of NaCl. The srlI gene was isolated from E. coli top10 and inserted it into E. coli DH5 alpha with pET32a+ vector was used. The confirmation of transgene integration and expression were made via the optical density (OD600) that is at 250 mmol/L NaCl concentration the srlI increase significantly (0.4227) compared with control (0.3564). The bacterial colonies with the recombined plasmid in the presence of IPTG and X-gal are colored white. The genotypic diversity of isolates was done using PCR that shows the similar patterns as the pattern of whole-cell protein for SDS-PAGE. Therefore, we need to attempt to explore the physiological mechanisms of E. coli srlI gene to salt tolerance in newly-developed transgenic bacteria lines for comparison of their physiological performance in a salt-stress environment.

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IJARIIE Ali Hussein Taher Alhilali. "Expression of the srlI gene to improve salt tolerance in Escherichia coli" International Journal Of Advance Research And Innovative Ideas In Education Volume 9 Issue 6 2023 Page 103-115
MLA Ali Hussein Taher Alhilali. "Expression of the srlI gene to improve salt tolerance in Escherichia coli." International Journal Of Advance Research And Innovative Ideas In Education 9.6(2023) : 103-115.
APA Ali Hussein Taher Alhilali. (2023). Expression of the srlI gene to improve salt tolerance in Escherichia coli. International Journal Of Advance Research And Innovative Ideas In Education, 9(6), 103-115.
Chicago Ali Hussein Taher Alhilali. "Expression of the srlI gene to improve salt tolerance in Escherichia coli." International Journal Of Advance Research And Innovative Ideas In Education 9, no. 6 (2023) : 103-115.
Oxford Ali Hussein Taher Alhilali. 'Expression of the srlI gene to improve salt tolerance in Escherichia coli', International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 6, 2023, p. 103-115. Available from IJARIIE, https://ijariie.com/AdminUploadPdf/Expression_of_the_srlI_gene_to_improve_salt_tolerance_in_Escherichia_coli_ijariie21871.pdf (Accessed : 03 November 2023).
Harvard Ali Hussein Taher Alhilali. (2023) 'Expression of the srlI gene to improve salt tolerance in Escherichia coli', International Journal Of Advance Research And Innovative Ideas In Education, 9(6), pp. 103-115IJARIIE [Online]. Available at: https://ijariie.com/AdminUploadPdf/Expression_of_the_srlI_gene_to_improve_salt_tolerance_in_Escherichia_coli_ijariie21871.pdf (Accessed : 03 November 2023)
IEEE Ali Hussein Taher Alhilali, "Expression of the srlI gene to improve salt tolerance in Escherichia coli," International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 6, pp. 103-115, Nov-Dec 2023. [Online]. Available: https://ijariie.com/AdminUploadPdf/Expression_of_the_srlI_gene_to_improve_salt_tolerance_in_Escherichia_coli_ijariie21871.pdf [Accessed : 03 November 2023].
Turabian Ali Hussein Taher Alhilali. "Expression of the srlI gene to improve salt tolerance in Escherichia coli." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 9 number 6 (03 November 2023).
Vancouver Ali Hussein Taher Alhilali. Expression of the srlI gene to improve salt tolerance in Escherichia coli. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2023 [Cited : 03 November 2023]; 9(6) : 103-115. Available from: https://ijariie.com/AdminUploadPdf/Expression_of_the_srlI_gene_to_improve_salt_tolerance_in_Escherichia_coli_ijariie21871.pdf
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