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Title: :  Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria
PaperId: :  20923
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 9 Issue 3 2023
DUI:    16.0415/IJARIIE-20923
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Nisha Shishodia Meerut Institute of Engineering anf Technology

Abstract

life science
UV-visible, FTIR, , antibacterial activity, Silver Nanoparticles, SEM, MIC , ZOI….
Nanoparticles are the center of attraction in recent years due to their immense application in electronics along with biological sciences as antimicrobial agents. The metallic nanoparticles remarkably differ in their physical and chemical properties from their corresponding bulk form and can be used as an anti-microbial agent. Green synthesis of nanomaterials is the environmentally friendly approach and most emerging method of nanoparticle manufacture. The present work utilized leaves of Azadirachta indica (neem), Punica granatum (pomegranate), Psidium guajava, Clove buds extract, and Ocimum sanctum (Tulsi) for reduction of 3mM AgNO3 to silver nanoparticles and compare the antimicrobial effects. The synthesis of nanoparticles was confirmed by the change of color from a colorless to a brown-colored solution. Further characterization was done by UV-visible spectroscopy and FTIR analysis, and UV-vis spectrophotometer characterized AgNPs with a range between 450–500 nm. The cellular structure of silver nanoparticles was studied by Phase contrast which gives confirmation that silver nanoparticles are in pure form. In the FTIR study, the stretching was found to be 617.18,1521.95,1122.49 and 2337.57. Well diffusion method showed the antimicrobial effect of AgNPs on different microorganisms with the zone of inhibition of 0.7, 0.6, 0.5,1 mm for Staphylococcus aureus, 0.8,0.9, 1.2, 0.9mm for Bacillus sp.0,8,0.9,1.2,1.1mm for Streptococcus, 0.8,0.6,1.3, for Pseudomonas and 1.1,1.4,1 0.9 1.3mm for E. coli under MIC 5mg/ml Mode of action of antimicrobial activity of nanoparticles was investigated by determining leakage of reducing sugars and proteins, suggesting that AgNPs were able to destroy membrane permeability. Out of all plants screened Guava is the best-reducing agent in terms of synthesis rate. Guava-mediated AgNPs were found to be highly active against microbes based on the zone of inhibition and minimal inhibitory concentration.

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IJARIIE Nisha Shishodia . "Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria" International Journal Of Advance Research And Innovative Ideas In Education Volume 9 Issue 3 2023 Page 4754-4765
MLA Nisha Shishodia . "Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria." International Journal Of Advance Research And Innovative Ideas In Education 9.3(2023) : 4754-4765.
APA Nisha Shishodia . (2023). Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria. International Journal Of Advance Research And Innovative Ideas In Education, 9(3), 4754-4765.
Chicago Nisha Shishodia . "Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria." International Journal Of Advance Research And Innovative Ideas In Education 9, no. 3 (2023) : 4754-4765.
Oxford Nisha Shishodia . 'Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria', International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 3, 2023, p. 4754-4765. Available from IJARIIE, https://ijariie.com/AdminUploadPdf/Comparative_analysis_of_the_Green_ijariie20923.pdf (Accessed : 02 August 2023).
Harvard Nisha Shishodia . (2023) 'Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria', International Journal Of Advance Research And Innovative Ideas In Education, 9(3), pp. 4754-4765IJARIIE [Online]. Available at: https://ijariie.com/AdminUploadPdf/Comparative_analysis_of_the_Green_ijariie20923.pdf (Accessed : 02 August 2023)
IEEE Nisha Shishodia , "Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria," International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 3, pp. 4754-4765, May-Jun 2023. [Online]. Available: https://ijariie.com/AdminUploadPdf/Comparative_analysis_of_the_Green_ijariie20923.pdf [Accessed : 02 August 2023].
Turabian Nisha Shishodia . "Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 9 number 3 (02 August 2023).
Vancouver Nisha Shishodia . Comparative analysis of the Green Synthesis of Silver nanoparticles using Azadirachta indica (neem ), Punica granatum (pomegranate), Psidium guajava, Clove buds extract and Ocimum sanctum (Tulsi) and their antimicrobial activity against pathogenic bacteria. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2023 [Cited : 02 August 2023]; 9(3) : 4754-4765. Available from: https://ijariie.com/AdminUploadPdf/Comparative_analysis_of_the_Green_ijariie20923.pdf
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