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Title: :  REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE
PaperId: :  21269
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 9 Issue 4 2023
DUI:    16.0415/IJARIIE-21269
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
DINAHARILALA Mamisitraka Théo. GloriatPlant Physiology Laboratory, unit : Plant Regeneration and micropropagation Laboratory, Faculty of Sciences, University of Antananarivo Madagascar
RAVONIARISON. NPlant Physiology Laboratory, unit : Plant Regeneration and micropropagation Laboratory, Faculty of Sciences, University of Antananarivo Madagascar

Abstract

Plant Biotechnology
in vitro germination, Leptolaena multiflora, mature zygotic embryo, regeneration
Leptolaena multiflora (L.) is endemic specie from Madagascar, renowned for its medicinal properties. It is listed as endangered on the IUCN red list. The seed surrounded by a hard shell is one of the limiting factors of its germination. This study aims to regenerate this specie by in vitro germination techniques and culture of mature zygotic embryo. The evaluation of the effects of types of seed scarification on germination shows that soaking seeds for 48 h improves the rate of seed germination. Besides, the increasing concentration of sucrose from 10 g/l to 40 g/l has a positive effect on the development of the embryo, and promotes the development of the aerial part and the root system of vitroplants. However, the high concentration above 80 g/l reduced the growth and in vitro development of the embryo. Beyond this dose, regeneration and root growth are weak and tend to stop.

Citations

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IJARIIE DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE" International Journal Of Advance Research And Innovative Ideas In Education Volume 9 Issue 4 2023 Page 2143-2151
MLA DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE." International Journal Of Advance Research And Innovative Ideas In Education 9.4(2023) : 2143-2151.
APA DINAHARILALA Mamisitraka Théo. Gloriat, & RAVONIARISON. N. (2023). REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE. International Journal Of Advance Research And Innovative Ideas In Education, 9(4), 2143-2151.
Chicago DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE." International Journal Of Advance Research And Innovative Ideas In Education 9, no. 4 (2023) : 2143-2151.
Oxford DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. 'REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE', International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 4, 2023, p. 2143-2151. Available from IJARIIE, https://ijariie.com/AdminUploadPdf/REGENERATION_OF_Leptolaena_multiflora__L___BY_IN_VITRO_SEED_GERMINATION_AND_ZYGOTIC_EMBRYO_CULTURE_ijariie21269.pdf (Accessed : ).
Harvard DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. (2023) 'REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE', International Journal Of Advance Research And Innovative Ideas In Education, 9(4), pp. 2143-2151IJARIIE [Online]. Available at: https://ijariie.com/AdminUploadPdf/REGENERATION_OF_Leptolaena_multiflora__L___BY_IN_VITRO_SEED_GERMINATION_AND_ZYGOTIC_EMBRYO_CULTURE_ijariie21269.pdf (Accessed : )
IEEE DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N, "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE," International Journal Of Advance Research And Innovative Ideas In Education, vol. 9, no. 4, pp. 2143-2151, Jul-Aug 2023. [Online]. Available: https://ijariie.com/AdminUploadPdf/REGENERATION_OF_Leptolaena_multiflora__L___BY_IN_VITRO_SEED_GERMINATION_AND_ZYGOTIC_EMBRYO_CULTURE_ijariie21269.pdf [Accessed : ].
Turabian DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. "REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 9 number 4 ().
Vancouver DINAHARILALA Mamisitraka Théo. Gloriat, and RAVONIARISON. N. REGENERATION OF Leptolaena multiflora (L.) BY IN VITRO SEED GERMINATION AND ZYGOTIC EMBRYO CULTURE. International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2023 [Cited : ]; 9(4) : 2143-2151. Available from: https://ijariie.com/AdminUploadPdf/REGENERATION_OF_Leptolaena_multiflora__L___BY_IN_VITRO_SEED_GERMINATION_AND_ZYGOTIC_EMBRYO_CULTURE_ijariie21269.pdf
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