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Title: :  In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)
PaperId: :  24772
Published in:   International Journal Of Advance Research And Innovative Ideas In Education
Publisher:   IJARIIE
e-ISSN:   2395-4396
Volume/Issue:    Volume 10 Issue 4 2024
DUI:    16.0415/IJARIIE-24772
Licence: :   IJARIIE is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author NameAuthor Institute
Abraham IsahUniversity of Abuja
Rebeccah Wusa NdanaUniversity of Abuja
Malann David YoilaUniversity of Abuja
Abdulrazak Baba IbrahimForum for Agricultural Research in Africa, Accra Ghana
Rose Suniso Maxwell GidadoNational Biotechnology Research and Development Agency
Lateef Ariyo AdeniranUniversity of Abuja

Abstract

Biotechnology and Bioinformatics
Transgene, In silico, Cry1Ab, PBR Cowpea, Allergenicity
Prior to the introduction of Genetically Engineered crops into the market, their safety needs to be thoroughly scrutinized for potential allergenicity and toxicity. The use of omics tool in safety assessment has been recognized as a critical tool for generating evidence of safety or otherwise of GE crops, by the Codex Alimentarius Guidelines of WHO and FAO. The objective of this study was to perform the safety analysis of PBR cowpea. For identification of the trait, lateral flow strip test using cry1Ab strips of lot number 6M1053 was used. PCR analysis was conducted for the amplification of the transgene. In addition, the morphological characteristics of the GE crop was evaluated using a non-transgenic cowpea as a standard. In silico analysis of the transgene product was performed to evaluate its three-dimensional x-ray crystal structure using fifteen Allergen and Toxin database. A total of 8,996,415 sequence alignment was conducted using BLASTP 2.2.27+, FASTA35.04 and BLOSUM62 scoring matrix to identify distant homologs. The cutoff for the e-value and maximum identity score was set at 1.0 and 35% respectively. The toxic or allergenic criteria were not met in all the database used, suggesting that the Cry1Ab transgene of the GM cowpea is safe for consumption. Analysis by sliding 80mer, sliding 8mer and 6 amino acid exact word-match also confirmed the transgene and its source organism are non-allergenic and risk-free. Evaluation of the morphological characteristics of the event also revealed that the transgene did not lead to any phenotypical alteration of the plant.

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IJARIIE Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. "In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)" International Journal Of Advance Research And Innovative Ideas In Education Volume 10 Issue 4 2024 Page 2352-2364
MLA Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. "In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)." International Journal Of Advance Research And Innovative Ideas In Education 10.4(2024) : 2352-2364.
APA Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, & Lateef Ariyo Adeniran. (2024). In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp). International Journal Of Advance Research And Innovative Ideas In Education, 10(4), 2352-2364.
Chicago Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. "In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)." International Journal Of Advance Research And Innovative Ideas In Education 10, no. 4 (2024) : 2352-2364.
Oxford Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. 'In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)', International Journal Of Advance Research And Innovative Ideas In Education, vol. 10, no. 4, 2024, p. 2352-2364. Available from IJARIIE, https://ijariie.com/AdminUploadPdf/In_silico_safety_assessment_and_morphological_characterization_of_transgenic_Maruca_vitrata_resistant_cowpea__Vigna_unguiculata_L__Walp__ijariie24772.pdf (Accessed : 30 October 2024).
Harvard Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. (2024) 'In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)', International Journal Of Advance Research And Innovative Ideas In Education, 10(4), pp. 2352-2364IJARIIE [Online]. Available at: https://ijariie.com/AdminUploadPdf/In_silico_safety_assessment_and_morphological_characterization_of_transgenic_Maruca_vitrata_resistant_cowpea__Vigna_unguiculata_L__Walp__ijariie24772.pdf (Accessed : 30 October 2024)
IEEE Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran, "In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)," International Journal Of Advance Research And Innovative Ideas In Education, vol. 10, no. 4, pp. 2352-2364, Jul-Aug 2024. [Online]. Available: https://ijariie.com/AdminUploadPdf/In_silico_safety_assessment_and_morphological_characterization_of_transgenic_Maruca_vitrata_resistant_cowpea__Vigna_unguiculata_L__Walp__ijariie24772.pdf [Accessed : 30 October 2024].
Turabian Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. "In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp)." International Journal Of Advance Research And Innovative Ideas In Education [Online]. volume 10 number 4 (30 October 2024).
Vancouver Abraham Isah, Rebeccah Wusa Ndana, Malann David Yoila, Abdulrazak Baba Ibrahim, Rose Suniso Maxwell Gidado, and Lateef Ariyo Adeniran. In silico safety assessment and morphological characterization of transgenic Maruca vitrata resistant cowpea (Vigna unguiculata L. Walp). International Journal Of Advance Research And Innovative Ideas In Education [Internet]. 2024 [Cited : 30 October 2024]; 10(4) : 2352-2364. Available from: https://ijariie.com/AdminUploadPdf/In_silico_safety_assessment_and_morphological_characterization_of_transgenic_Maruca_vitrata_resistant_cowpea__Vigna_unguiculata_L__Walp__ijariie24772.pdf
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