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In the analysis of a PKL it is shown that the Major and minor acids present in dried leaf tissue of Bryophyllum calycinum were identified or characterized using the combined methods of ion exchange chromatography, paper and thin-layer chromatography, gas chromatography, and infrared spectroscopy. After esterification of the acids in methanol containing H+ form cation exchange resin, their amounts were determined by gas chromatography. The acids identified and their approximate percentage amounts of the total acid content were: malic 32.5%, citric 10.1%, isocitric 46.5%, succinic 1.0%, fumaric 0.9%, pyruvic 1.0%, oxalacetic 0.4%, α-ketoglutaric 0.5%, glyoxylic 0.1%, lactic 0.2%, oxalic 0.2%, andcis-aconitic 1.6%. Tentative characteristics were proposed for previously undetermined acids present mainly in amounts from 0.05% to 0.6%. These were apparently mono-, di-, and tricarboxylic acids containing from three to seven carbons in the carbon chain. Most of these evidently had additional functional groups, including methyl, keto, and hydroxyl groups. The boiling points of the methyl esters of these acids ranged from 140°C to 300°C. It is seen experimentally that there are seventeen elements in the plant which helps to grow their full physiology. These are: Carbon, Hydrogen, Oxygen, Nitrogen, Potassium, Calcium, Phosphorous, Corpur, Magnesium, Iron, Manganese, Zinc, Copper, Boron, Molybdenum, Sodium and Chlorine. Plant absorbs carbon, Hydrogen and Oxygen except rest of the 14 elements. Furthermore, plant absorbs 10 types of cation and 7 types of anion. The solution of the sand has adequately Ca++ , mg++ , k+ , Na+ , H+ as a cation and cl- , So4-- , HCo3-, NO-3 and OH- as anion. But the rate of absorption of ions is different. The plasma membrane of the cell is not allowed to penetrable for all ions. It is considered that k+ and NO3- absorbed rapidly. On the other hand the rate of absorption Ca++ and SO4-- is slow. To keep it in mind we have studied the variation of pH with time during day and night time |